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验证数据展示

  • WB analysis of A375 using 66426-1-Ig (same clone as 66426-1-PBS)

    WB analysis of A375 using 66426-1-Ig (same clone as 66426-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 66426-1-Ig (SPARC Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66426-1-Ig (same clone as 66426-1-PBS)

    IHC staining of human placenta using 66426-1-Ig (same clone as 66426-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66426-1-Ig (SPARC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPARC antibody included
  • IHC staining of human placenta using 66426-1-Ig (same clone as 66426-1-PBS)

    IHC staining of human placenta using 66426-1-Ig (same clone as 66426-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66426-1-Ig (SPARC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPARC antibody included
  • IF Staining of C6 using 66426-1-Ig (same clone as 66426-1-PBS)

    IF Staining of C6 using 66426-1-Ig (same clone as 66426-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using SPARC antibody (66426-1-Ig, Clone: 1A2C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

SPARC Monoclonal antibody, PBS Only

SPARC Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66426-1-PBS

产品说明书

CloneNo. 1A2C1

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Basement membrane protein 40, BM 40, ON, Osteonectin, SPARC

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SPARC Ready-To-Use IHC Kit. SPARC primary antibody included.

产品信息

66426-1-PBS targets SPARC in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 SPARC fusion protein Ag7390 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 secreted protein, acidic, cysteine-rich (osteonectin)
别名 Basement membrane protein 40, BM 40, ON, Osteonectin, SPARC
计算分子量 35 kDa
观测分子量 38 kDa, 52 kDa
GenBank蛋白编号BC004974
基因名称 SPARC
Gene ID (NCBI) 6678
RRIDAB_2881797
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09486
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).