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SPARC Recombinant antibody, PBS Only (Capture)

SPARC Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241952A12

Cat no : 84621-1-PBS

Print datasheet

Synonyms

ON, BM-40, BM 40, Basement-membrane protein 40, Basement membrane protein 40

验证数据展示

  • Sandwich ELISA standard curve of MP01430-2

    Sandwich ELISA standard curve of MP01430-2

    Sandwich ELISA standard curve of MP01430-2, Human SPARC Recombinant Matched Antibody Pair - PBS only. 84621-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1169. 84621-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-1250 pg/mL

  • Sample test of MP01430-2

    Sample test of MP01430-2

    Serum of four healthy human donors was measured. The human SPARC concentration of detected samples was determined to be 212,811.0 pg/mL with a range of 153,761.1 - 333,381.8 pg/mL.

  • Sample test of MP01430-2

    Sample test of MP01430-2

    MG-63 cells were cultured in DMEM supplemented with 10% fetal bonive serum. An aliquot of the cell culture supernate was removed, assayed for human SPARC, and measured 91,240.8 pg/mL.

  • Sample test of MP01430-2

    Sample test of MP01430-2

    Human milk of four healthy human donors was measured. The human SPARC concentration of detected samples was determined to be 1,084.0 pg/mL with a range of 425.0 - 1,594.2 pg/mL.

  • Cytometric bead array standard curve of MP01430-1

    Cytometric bead array standard curve of MP01430-1

    Cytometric bead array standard curve of MP01430-1, SPARC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84621-1-PBS. Detection antibody: 84621-2-PBS. Standard: Eg1169. Range: 0.313-10 ng/mL.

  • Cytometric bead array standard curve of MP01430-2

    Cytometric bead array standard curve of MP01430-2

    Cytometric bead array standard curve of MP01430-2, SPARC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84621-1-PBS. Detection antibody: 84621-3-PBS. Standard: Eg1169. Range: 0.313-10 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy SPARC Ready-To-Use IHC Kit. SPARC primary antibody included.

产品信息

84621-1-PBS targets SPARC as part of a matched antibody pair:

MP01430-1: 84621-1-PBS capture and 84621-2-PBS detection (validated in Cytometric bead array)

MP01430-2: 84621-1-PBS capture and 84621-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name secreted protein, acidic, cysteine-rich (osteonectin)
Synonyms ON, BM-40, BM 40, Basement-membrane protein 40, Basement membrane protein 40
Calculated Molecular Weight35kDa
GenBank Accession NumberNM_003118.4
Gene Symbol SPARC
Gene ID (NCBI) 6678
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09486
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C