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SPAST Polyclonal antibody
SPAST Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, PC-3 cells |
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
22792-1-AP targets SPAST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | SPAST fusion protein Ag18797 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | spastin |
Synonyms | spastin, EC:5.6.1.1 |
Calculated Molecular Weight | 616 aa, 67 kDa |
Observed Molecular Weight | 52 kDa |
GenBank Accession Number | BC150260 |
Gene Symbol | SPAST |
Gene ID (NCBI) | 6683 |
RRID | AB_2879170 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9UBP0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SPAST antibody 22792-1-AP | Download protocol |
IHC protocol for SPAST antibody 22792-1-AP | Download protocol |
IF protocol for SPAST antibody 22792-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Rep Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.
| ||
FASEB J Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane. | ||
Mol Biol Cell Spastin regulates anaphase chromosome separation distance and microtubule-containing nuclear tunnels | ||
Acta Neuropathol Commun The enhanced association between mutant CHMP2B and spastin is a novel pathological link between frontotemporal dementia and hereditary spastic paraplegias |