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  • KD/KO Validated

SPAST Polyclonal antibody

SPAST Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 22792-1-AP

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Synonyms

spastin, EC:5.6.1.1



经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22792-1-AP targets SPAST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen SPAST fusion protein Ag18797 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name spastin
Synonyms spastin, EC:5.6.1.1
Calculated Molecular Weight 616 aa, 67 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC150260
Gene Symbol SPAST
Gene ID (NCBI) 6683
RRIDAB_2879170
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBP0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPAST encodes a microtubule-severing protein called spastin. By severing microtubules into small segments, SPAST participates in the growth and regeneration of neurites. Mutations in the SPAST gene (previously known as SPG4) are the most common causes of hereditary spastic paraplegia (HSP-SPG4). Mammalian cells express at least two spastin isoforms, a less-abundant full-length form (M1-spastin, 68 kDa) and a more highly expressed isoform (M87-spastin, 60 kDa) that lacks the N-terminal 86 amino acids of the full-length protein. Also, there are two additional 64 kDa and 55 kDa isoforms due to alternative splicing of SPG4 exon 4. This antibody recognizes all these isoforms of spastin.

实验方案

Product Specific Protocols
WB protocol for SPAST antibody 22792-1-APDownload protocol
IHC protocol for SPAST antibody 22792-1-APDownload protocol
IF protocol for SPAST antibody 22792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Authors - Inna Goliand
  • KO Validated
ratWB

FASEB J

Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane.

Authors - Ying Gao
humanWB

Mol Biol Cell

Spastin regulates anaphase chromosome separation distance and microtubule-containing nuclear tunnels

Authors - Megan E Kelley
human,mouseWB,IF

Acta Neuropathol Commun

The enhanced association between mutant CHMP2B and spastin is a novel pathological link between frontotemporal dementia and hereditary spastic paraplegias

Authors - Yongping Chen