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SPATA13 Polyclonal antibody

SPATA13 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21400-1-AP

Print datasheet

Synonyms

Spermatogenesis-associated protein 13, spermatogenesis associated 13, Asef2

验证数据展示

  • WB analysis of COLO 320 using 21400-1-AP

    WB analysis of COLO 320 using 21400-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21400-1-AP (SPATA13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 21400-1-AP

    IF Staining of HepG2 using 21400-1-AP

    Immunofluorescent analysis of HepG2 cells, using SPATA13 antibody 21400-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inCOLO 320 cells, human placenta tissue
Positive IF/ICC detected inHepG2 cells, HEK-293 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21400-1-AP targets SPATA13 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SPATA13 fusion protein Ag16101 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name spermatogenesis associated 13
Synonyms Spermatogenesis-associated protein 13, spermatogenesis associated 13, Asef2
Calculated Molecular Weight 652 aa, 75 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC109291
Gene Symbol SPATA13
Gene ID (NCBI) 221178
RRIDAB_10859783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96N96
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPATA13 antibody 21400-1-APDownload protocol
IF protocol for SPATA13 antibody 21400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols