验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, human testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SPATA22 Ready-To-Use IHC Kit. SPATA22 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16989-1-AP targets SPATA22 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | SPATA22 fusion protein Ag10591 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spermatogenesis associated 22 |
| 别名 | NYD SP20, SPATA22, spermatogenesis associated 22 |
| 计算分子量 | 363 aa, 41 kDa |
| 观测分子量 | 41 kDa |
| GenBank蛋白编号 | BC029483 |
| 基因名称 | SPATA22 |
| Gene ID (NCBI) | 84690 |
| RRID | AB_2286640 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NHS9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Spata22 (NYD-SP20), a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells. It is essential for one or more key events of early meiotic prophase, as homologous chromosomes of mutant germ cells do not achieve normal synapsis or repair meiotic DNA double-strand breaks.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPATA22 antibody 16989-1-AP | Download protocol |
| IHC protocol for SPATA22 antibody 16989-1-AP | Download protocol |
| IP protocol for SPATA22 antibody 16989-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis | ||
Nat Commun Nutrient restriction synergizes with retinoic acid to induce mammalian meiotic initiation in vitro. | ||
Nat Commun The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells. | ||
Nucleic Acids Res SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination. | ||
Cell Death Differ A truncating variant of RAD51B associated with primary ovarian insufficiency provides insights into its meiotic and somatic functions. | ||
Nat Commun MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination.
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