• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of THP-1 using 26474-1-AP

    WB analysis of THP-1 using 26474-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 26474-1-AP (SPC25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 26474-1-AP

    IHC staining of mouse spleen using 26474-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26474-1-AP (SPC25 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 26474-1-AP

    IHC staining of mouse spleen using 26474-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26474-1-AP (SPC25 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

SPC25 Polyclonal antibody

SPC25 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26474-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

AD024, hSpc25, Kinetochore protein Spc25, SPBC25, SPC25

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inTHP-1 cells, HL-60 cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26474-1-AP targets SPC25 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 SPC25 fusion protein Ag23985 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae)
别名 AD024, hSpc25, Kinetochore protein Spc25, SPBC25, SPC25
观测分子量 26 kDa
GenBank蛋白编号BC022255
基因名称 SPC25
Gene ID (NCBI) 57405
RRIDAB_2880530
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBM1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPC25 antibody 26474-1-APDownload protocol
IHC protocol for SPC25 antibody 26474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Aging (Albany NY)

SPC25 overexpression promotes tumor proliferation and is prognostic of poor survival in hepatocellular carcinoma.

Authors - Baozhu Zhang
View Article
humanWB

Cell Biol Int

PLEK2 activates the PI3K/AKT signaling pathway to drive lung adenocarcinoma progression by upregulating SPC25

Authors - Wenqian Zhang
View Article
humanWB,IHC

JTO Clin Res Rep

CADM1 and SPC25 Gene Mutations in Lung Cancer Patients With Idiopathic Pulmonary Fibrosis.

Authors - Aya Fukuizumi
  • KD Validated
View Article