验证数据展示
经过测试的应用
Positive IHC detected in | mouse skeletal muscle tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18716-1-AP targets SPEG in IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | IHC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | SPEG complex locus |
别名 | APEG 1, APEG1, BPEG, KIAA1297, SPEG, SPEG complex locus, SPEGalpha, SPEGbeta |
计算分子量 | 354 kDa |
GenBank蛋白编号 | NM_005876 |
基因名称 | SPEG |
Gene ID (NCBI) | 10290 |
RRID | AB_3085566 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q15772 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPEG, also named as BPEG, APEG1 and KIAA1297, is highly similar to the corresponding rat and mouse proteins. Expression of SPEG is thought to serve as a marker for differentiated vascular smooth muscle cells which may have a role in regulating growth and differentiation of this cell type. SPEG is expressed in vascular smooth muscle cells and is down-regulated by vascular injury. Multiple transcripts are produced from the SPEG complex locus by alternative splicing and alternative promoter usage. The antibody recognizes the C-term of SPEG.
实验方案
Product Specific Protocols | |
---|---|
IHC protocol for SPEG antibody 18716-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |