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验证数据展示

  • WB analysis using 27801-1-AP

    WB analysis using 27801-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27801-1-AP (SPG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 27801-1-AP

    IP experiment of HeLa using 27801-1-AP

    IP result of anti-SPG7 (IP:27801-1-AP, 4ug; Detection:27801-1-AP 1:500) with HeLa cells lysate 2800 ug.

  • IHC staining of human liver using 27801-1-AP

    IHC staining of human liver using 27801-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27801-1-AP (SPG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 27801-1-AP

    IHC staining of human liver using 27801-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27801-1-AP (SPG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SPG7 Polyclonal antibody

SPG7 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 27801-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IP, IHC, ELISA

别名

CAR, CMAR, Paraplegin, PGN, Spastic paraplegia 7 protein, SPG5C, SPG7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, LNCaP cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27801-1-AP targets SPG7 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 SPG7 fusion protein Ag26265 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spastic paraplegia 7 (pure and complicated autosomal recessive)
别名 CAR, CMAR, Paraplegin, PGN, Spastic paraplegia 7 protein, SPG5C, SPG7
计算分子量 88 kDa
观测分子量54 kDa, 70 kDa, 80kDa
GenBank蛋白编号BC007692
基因名称 SPG7
Gene ID (NCBI) 6687
RRIDAB_2918133
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQ90
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPG7 encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA protein family. Two transcript variants encoding distinct isoforms have been identified. The molecular weight of SPG7 is about 88-100 kDa (precursor),80 kDa (intermediate) and 70 kDa (mature)(PMID: 24767997), while the molecular weight of isoform 2 is about 54 kDa.

实验方案

Product Specific Protocols
WB protocol for SPG7 antibody 27801-1-APDownload protocol
IHC protocol for SPG7 antibody 27801-1-APDownload protocol
IP protocol for SPG7 antibody 27801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols