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验证数据展示

  • WB analysis using 27801-1-AP

    WB analysis using 27801-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27801-1-AP (SPG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 27801-1-AP

    IP experiment of HeLa using 27801-1-AP

    IP result of anti-SPG7 (IP:27801-1-AP, 4ug; Detection:27801-1-AP 1:500) with HeLa cells lysate 2800 ug.

  • IHC staining of human liver using 27801-1-AP

    IHC staining of human liver using 27801-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27801-1-AP (SPG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 27801-1-AP

    IHC staining of human liver using 27801-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27801-1-AP (SPG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SPG7 Polyclonal antibody

SPG7 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 27801-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IP, IHC, ELISA

Synonyms

CAR, CMAR, Paraplegin, PGN, Spastic paraplegia 7 protein, SPG5C, SPG7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, LNCaP cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27801-1-AP targets SPG7 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen SPG7 fusion protein Ag26265 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name spastic paraplegia 7 (pure and complicated autosomal recessive)
Synonyms CAR, CMAR, Paraplegin, PGN, Spastic paraplegia 7 protein, SPG5C, SPG7
Calculated Molecular Weight 88 kDa
Observed Molecular Weight54 kDa, 70 kDa, 80kDa
GenBank Accession NumberBC007692
Gene Symbol SPG7
Gene ID (NCBI) 6687
RRIDAB_2918133
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQ90
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPG7 encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA protein family. Two transcript variants encoding distinct isoforms have been identified. The molecular weight of SPG7 is about 88-100 kDa (precursor),80 kDa (intermediate) and 70 kDa (mature)(PMID: 24767997), while the molecular weight of isoform 2 is about 54 kDa.

实验方案

Product Specific Protocols
WB protocol for SPG7 antibody 27801-1-APDownload protocol
IHC protocol for SPG7 antibody 27801-1-APDownload protocol
IP protocol for SPG7 antibody 27801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols