验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells, MCF-7 cells, NIH/3T3 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82609-1-RR targets SPHK1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | SPHK1 fusion protein Ag1051 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | sphingosine kinase 1 |
| 别名 | Sphingosine kinase 1, SK1, EC:2.7.1.91, EC:2.3.1.-, Acetyltransferase SPHK1 |
| 计算分子量 | 43 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC008040 |
| 基因名称 | SPHK1 |
| Gene ID (NCBI) | 8877 |
| RRID | AB_3086491 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NYA1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPHK1 antibody 82609-1-RR | Download protocol |
| IF protocol for SPHK1 antibody 82609-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |