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  • Featured Product
  • KD/KO Validated

SPHK2 Polyclonal antibody, PBS Only

SPHK2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17096-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 17096-1-AP (same clone as 17096-1-PBS)

    WB analysis using 17096-1-AP (same clone as 17096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • WB analysis using 17096-1-AP (same clone as 17096-1-PBS)

    WB analysis using 17096-1-AP (same clone as 17096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 17096-1-AP (same clone as 17096-1-PBS)

    WB analysis of A549 using 17096-1-AP (same clone as 17096-1-PBS)

    WB result of SPHK2 antibody (17096-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPHK2 transfected A549 cells. This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • WB analysis using 17096-1-AP (same clone as 17096-1-PBS)

    WB analysis using 17096-1-AP (same clone as 17096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 17096-1-AP (same clone as 17096-1-PBS)

    IP experiment of mouse liver using 17096-1-AP (same clone as 17096-1-PBS)

    IP result of anti-SPHK2 (IP:17096-1-AP, 4ug; Detection:17096-1-AP 1:800) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 17096-1-AP (same clone as 17096-1-PBS)

    IHC staining of human breast cancer using 17096-1-AP (same clone as 17096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPHK2 antibody included
  • IHC staining of human breast cancer using 17096-1-AP (same clone as 17096-1-PBS)

    IHC staining of human breast cancer using 17096-1-AP (same clone as 17096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPHK2 antibody included
  • IHC staining of human kidney using 17096-1-AP (same clone as 17096-1-PBS)

    IHC staining of human kidney using 17096-1-AP (same clone as 17096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPHK2 antibody included
  • IHC staining of human kidney using 17096-1-AP (same clone as 17096-1-PBS)

    IHC staining of human kidney using 17096-1-AP (same clone as 17096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPHK2 antibody included
  • WB analysis using 17096-1-AP (same clone as 17096-1-PBS)

    WB analysis using 17096-1-AP (same clone as 17096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 17096-1-AP (same clone as 17096-1-PBS)

    IF Staining of HeLa using 17096-1-AP (same clone as 17096-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPHK2 antibody (17096-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 17096-1-AP (same clone as 17096-1-PBS)

    FC experiment of HeLa using 17096-1-AP (same clone as 17096-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SPHK2 (17096-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SPHK2 Ready-To-Use IHC Kit. SPHK2 primary antibody included.

产品信息

17096-1-PBS targets SPHK2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SPHK2 fusion protein Ag10569 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sphingosine kinase 2
Synonyms
Calculated Molecular Weight 618 aa, 65 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC010671
Gene Symbol SPHK2
Gene ID (NCBI) 56848
RRIDAB_10598479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRA0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPHK2(Sphingosine kinase 2) is also named as SK2, SPK2 and catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK2 associates with HDAC1 and HDAC2 in repressor complexes and is selectively enriched at the promoters of the genes encoding the cyclin-dependent kinase inhibitor p21 or the transcriptional regulator c-fos, where it enhanced local histone H3 acetylation and transcription.SphK2 is a ~66kDa protein that is most abundantly expressed in the liver and heart(PMID:19168577). SphK2 has two splice variants, which are the 68-kDa SphK2-S and the NH2-terminal extended 72-kDa SphK2-L (PMID:17974990).