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SPIN2B Polyclonal antibody

SPIN2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10107-2-AP

Print datasheet

Synonyms

SPIN-2B, SPIN2_duplicate, SPIN-2, SPIN2, SPIN 2B

验证数据展示

  • WB analysis of mouse brain using 10107-2-AP

    WB analysis of mouse brain using 10107-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10107-2-AP (SPIN2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10107-2-AP

    IHC staining of human breast cancer using 10107-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 10107-2-AP

    IHC staining of human colon using 10107-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 10107-2-AP

    IF Staining of HeLa using 10107-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SPIN2B antibody (10107-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10107-2-AP targets SPIN2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SPIN2B fusion protein Ag0157 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name spindlin family, member 2B
Synonyms SPIN-2B, SPIN2_duplicate, SPIN-2, SPIN2, SPIN 2B
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC000044
Gene Symbol SPIN2B
Gene ID (NCBI) 474343
RRIDAB_2196120
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BPZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPIN2B antibody 10107-2-APDownload protocol
IHC protocol for SPIN2B antibody 10107-2-APDownload protocol
IF protocol for SPIN2B antibody 10107-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols