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验证数据展示

  • WB analysis of mouse brain using 10107-2-AP

    WB analysis of mouse brain using 10107-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10107-2-AP (SPIN2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10107-2-AP

    IHC staining of human breast cancer using 10107-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 10107-2-AP

    IHC staining of human colon using 10107-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 10107-2-AP

    IF Staining of HeLa using 10107-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SPIN2B antibody (10107-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

SPIN2B Polyclonal antibody

SPIN2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10107-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

SPIN-2B, SPIN2_duplicate, SPIN-2, SPIN2, SPIN 2B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10107-2-AP targets SPIN2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SPIN2B fusion protein Ag0157 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spindlin family, member 2B
别名 SPIN-2B, SPIN2_duplicate, SPIN-2, SPIN2, SPIN 2B
计算分子量 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC000044
基因名称 SPIN2B
Gene ID (NCBI) 474343
RRIDAB_2196120
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BPZ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPIN2B antibody 10107-2-APDownload protocol
IHC protocol for SPIN2B antibody 10107-2-APDownload protocol
IF protocol for SPIN2B antibody 10107-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols