验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse testis tissue, A549 cells, mouse epididymis tissue, rat testis tissue |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31931-1-AP targets SPINT3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | SPINT3 fusion protein Ag35289 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serine peptidase inhibitor, Kunitz type, 3 |
| 别名 | HKIB9, Kunitz-type protease inhibitor 3 |
| 计算分子量 | 10 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | NM_006652.1 |
| 基因名称 | SPINT3 |
| Gene ID (NCBI) | 10816 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P49223 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Serine peptidase inhibitor, Kunitz type 3 (SPINT3, also known as HKIB9) might be related to serine-type endopeptidase inhibitor activity, receptor antagonist activity, and transforming growth factor beta binding (PMID: 21988899). The calculated molecular weight of SPINT3 is 10 kDa, which is lower than its observed molecular weight of 30-35 kDa by SDS-PAGE detection (PMID: 21988899).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPINT3 antibody 31931-1-AP | Download protocol |
| IF protocol for SPINT3 antibody 31931-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |