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验证数据展示

  • WB analysis using 11295-1-AP

    WB analysis using 11295-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11295-1-AP (SPIRE1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 11295-1-AP

    IHC staining of human lung cancer using 11295-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11295-1-AP (SPIRE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .

  • IHC staining of human lung cancer using 11295-1-AP

    IHC staining of human lung cancer using 11295-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11295-1-AP (SPIRE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

SPIRE1 Polyclonal antibody

SPIRE1 Polyclonal Antibody for IHC, ELISA
Cat No. 11295-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

KIAA1135, Protein spire homolog 1, Spir 1, SPIR1, spire homolog 1 (Drosophila), SPIRE1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11295-1-AP targets SPIRE1 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原 SPIRE1 fusion protein Ag1798 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spire homolog 1 (Drosophila)
别名 KIAA1135, Protein spire homolog 1, Spir 1, SPIR1, spire homolog 1 (Drosophila), SPIRE1
计算分子量 64 kDa
观测分子量50-60 kDa, 95 kDa
GenBank蛋白编号BC016825
基因名称 SPIRE1
Gene ID (NCBI) 56907
RRIDAB_3085370
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ08AE8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPIRE1, Protein spire homolog 1, belongs to the family of Wiskott-Aldrich homology region-2 (WH2) proteins (PMID: 11747823). SPIRE1 acts as an actin nucleation factor, associated with the slow-growing pointed end of the new filament (PMID: 11747823, PMID: 21620703). SPIRE1 and FMN2 promote assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PMID: 26287480).

实验方案

Product Specific Protocols
WB protocol for SPIRE1 antibody 11295-1-APDownload protocol
IHC protocol for SPIRE1 antibody 11295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols