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- KD/KO Validated
PBK Polyclonal antibody
PBK Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, FC (Intra), ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, mouse ovary tissue, mouse testis tissue, NIH/3T3 cells, rat testis tissue |
| Positive IHC detected in | human placenta tissue, human lung cancer tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy PBK Ready-To-Use IHC Kit. PBK primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16110-1-AP targets PBK in WB, IHC, FC (Intra), IP, CoIP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, FC (Intra), ELISA Application Description |
| Cited Applications | WB, IHC, IP, CoIP, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | PBK fusion protein Ag9033 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | PDZ binding kinase |
| Synonyms | MAPKK-like protein kinase, MAPKK like protein kinase, Lymphokine-activated killer T-cell-originated protein kinase, EC:2.7.12.2, CT84 |
| Calculated Molecular Weight | 322 aa, 36 kDa |
| Observed Molecular Weight | 36-40 kDa |
| GenBank Accession Number | BC015191 |
| Gene Symbol | PBK |
| Gene ID (NCBI) | 55872 |
| RRID | AB_2283508 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96KB5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PBK(PDZ-binding kinase) is also named as TOPK, CT84, Nori-3, SPK and belongs to the MAP kinase kinase subfamily. PBK may have a role in the regulation of cellular proliferation and progression of the cell cycle. It has a characteristic cdc2ycyclin B phosphorylation site(SyT-P-X-KyR) at its N terminus,which is conserved across species, and it is phosphorylated in a cell cycle-dependent manner at mitosis, and that this phosphorylation is required for its activation(PMID:10779557).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PBK antibody 16110-1-AP | Download protocol |
| IHC protocol for PBK antibody 16110-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genomics Proteomics Bioinformatics Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma. | ||
Invest Ophthalmol Vis Sci Single-Cell Transcriptomics Reveals Cellular Heterogeneity and Complex Cell-Cell Communication Networks in the Mouse Cornea | ||
Biomolecules Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods | ||
Invest Ophthalmol Vis Sci Single-Cell RNA Transcriptome Helps Define the Limbal/Corneal Epithelial Stem/Early Transit Amplifying Cells and How Autophagy Affects This Population.
| ||
Front Endocrinol (Lausanne) PBK/TOPK Inhibitor Suppresses the Progression of Prolactinomas. |
