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SPOCD1 Polyclonal antibody

SPOCD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22243-1-AP

Print datasheet

Synonyms

RP11 84A19.1, SPOC domain containing 1, SPOCD1

验证数据展示

  • WB analysis of SH-SY5Y using 22243-1-AP

    WB analysis of SH-SY5Y using 22243-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inSH-SY5Y cells, HEK-293 cells, human testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

22243-1-AP targets SPOCD1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen SPOCD1 fusion protein Ag17734 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SPOC domain containing 1
Synonyms RP11 84A19.1, SPOC domain containing 1, SPOCD1
Calculated Molecular Weight 1203 aa, 129 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC114430
Gene Symbol SPOCD1
Gene ID (NCBI) 90853
RRIDAB_2879045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ6ZMY3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The PIWI protein MIWI2 (PIWIL4; 610315) and its associated PIWI-interacting RNAs (piRNAs) direct DNA methylation of transposable elements in the male germline. SPOCD1 is an essential nuclear effector of MIWI2- and piRNA-directed DNA methylation (PubMed: 32674113).

实验方案

Product Specific Protocols
WB protocol for SPOCD1 antibody 22243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncotarget

Bromodomain protein BRD4 inhibitor JQ1 regulates potential prognostic molecules in advanced renal cell carcinoma.

Authors - Takashi Sakaguchi
View Article