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验证数据展示

  • WB analysis using 28203-1-AP

    WB analysis using 28203-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28203-1-AP (SPOCK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 28203-1-AP

    IHC staining of human prostate cancer using 28203-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28203-1-AP (SPOCK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 28203-1-AP

    IHC staining of human prostate cancer using 28203-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28203-1-AP (SPOCK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SPOCK1 Polyclonal antibody

SPOCK1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28203-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human and More (1)

应用

WB, IHC, ELISA

别名

FLJ37170, Protein SPOCK, SPOCK, SPOCK1, TESTICAN, Testican 1, TIC1, TICN1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells, DU 145 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28203-1-AP targets SPOCK1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human
文献引用反应性human
免疫原 SPOCK1 fusion protein Ag28213 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1
别名 FLJ37170, Protein SPOCK, SPOCK, SPOCK1, TESTICAN, Testican 1, TIC1, TICN1
计算分子量 439 aa, 49 kDa
观测分子量49 kDa
GenBank蛋白编号BC030691
基因名称 SPOCK1
Gene ID (NCBI) 6695
RRIDAB_3086036
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ08629
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPOCK1 antibody 28203-1-APDownload protocol
IHC protocol for SPOCK1 antibody 28203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Heliyon

Extrachromosomal circular DNA-related SPOCK1 contributes to development and enzalutamide resistance of prostate cancer by regulating epithelial mesenchymal transition

Authors - Yicong Yao
View Article