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验证数据展示

  • WB analysis using 84977-1-RR

    WB analysis using 84977-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84977-1-RR (SPOCK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.ce

  • Affinity and Kinetic Characterization of 84977-1-RR

    Affinity and Kinetic Characterization of 84977-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84977-1-RR against Human SPOCK1 were performed. The affinity constant is 3.51 nM.

SPOCK1 Recombinant antibody

SPOCK1 Recombinant Antibody for WB, ELISA
Cat No. 84977-1-RR

产品说明书

CloneNo. 242196E10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Testican-1, Testican 1, TESTICAN, SPOCK, Protein SPOCK

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDU 145 cells, PC-3 eclls, HEK-293T cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84977-1-RR targets SPOCK1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 SPOCK1 fusion protein Ag28204 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1
别名 Testican-1, Testican 1, TESTICAN, SPOCK, Protein SPOCK
计算分子量 439 aa, 49 kDa
观测分子量49 kDa
GenBank蛋白编号BC030691
基因名称 SPOCK1
Gene ID (NCBI) 6695
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ08629
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPOCK1, also known as TICN1. It is predicted to be located in the extracellular matrix. The proein may play a role in cell-cell and cell-matrix interactions and may contribute to various neuronal mechanisms in the central nervous system. The molecular weight of SPOCK1 is 49 kDa.

实验方案

Product Specific Protocols
WB protocol for SPOCK1 antibody 84977-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols