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SPOPL Polyclonal antibody

SPOPL Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17740-1-AP

Print datasheet

Synonyms

HIB homolog 2, Roadkill homolog 2, speckle type POZ protein like, SPOPL

验证数据展示

  • WB analysis using 17740-1-AP

    WB analysis using 17740-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17740-1-AP (SPOPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17740-1-AP targets SPOPL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SPOPL fusion protein Ag11951 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name speckle-type POZ protein-like
Synonyms HIB homolog 2, Roadkill homolog 2, speckle type POZ protein like, SPOPL
Calculated Molecular Weight 392 aa, 45 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberBC071613
Gene Symbol SPOPL
Gene ID (NCBI) 339745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6IQ16
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Speckle Type POZ Protein Like (SPOPL), a member of the MATH-BTB protein family, was frst identifed in 2010 with 82.6% sequence homology with Speckle-type POZ protein (SPOP). SPOPL is an E3 ubiquitin ligase protein that ubiquitinates and degrades the target protein. SPOPL can be used as a potential prognostic biomarker for glioblastoma multiforme in clinical work and promotes the proliferation and stemness of glioma stem cells by activating the Notch signaling pathway (PMID:37523046).

实验方案

Product Specific Protocols
WB protocol for SPOPL antibody 17740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols