验证数据展示
经过测试的应用
Positive IHC detected in | human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 2 publications below |
产品信息
11959-1-AP targets SPRR1B in IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | IHC, ELISA Application Description |
文献引用应用 | IHC |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | SPRR1B fusion protein Ag2563 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | small proline-rich protein 1B (cornifin) |
别名 | 14.9 kDa pancornulin, CORNIFIN, Cornifin B, GADD33, Small proline rich protein IB, SPR IB, SPRR1, SPRR1B |
计算分子量 | 89 aa, 10 kDa |
GenBank蛋白编号 | BC056240 |
基因名称 | SPRR1B |
Gene ID (NCBI) | 6699 |
RRID | AB_2877807 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P22528 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This protein functions as a component of the cross-linked envelope in squamous differentiating cells. It runs as a 15-kDa protein in unreducing SDS PAGE, whereas under reducing conditions it runs as 23 kDa one.
实验方案
Product Specific Protocols | |
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IHC protocol for SPRR1B antibody 11959-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Discov Single-cell atlas of keratoconus corneas revealed aberrant transcriptional signatures and implicated mechanical stretch as a trigger for keratoconus pathogenesis. | ||
Front Oncol Long Noncoding RNA HAGLROS Promotes the Malignant Progression of Bladder Cancer by Regulating the miR-330-5p/SPRR1B Axis. |