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验证数据展示

  • WB analysis of HeLa using 11742-1-AP (same clone as 11742-1-PBS)

    WB analysis of HeLa using 11742-1-AP (same clone as 11742-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11742-1-AP (SPRR3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11742-1-AP (same clone as 11742-1-PBS)

    IHC staining of human colon cancer using 11742-1-AP (same clone as 11742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11742-1-AP (same clone as 11742-1-PBS)

    IHC staining of human colon cancer using 11742-1-AP (same clone as 11742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • IP experiment of COLO 320 using 11742-1-AP (same clone as 11742-1-PBS)

    IP experiment of COLO 320 using 11742-1-AP (same clone as 11742-1-PBS)

    IP result of anti-SPRR3 (IP:11742-1-AP, 3ug; Detection:11742-1-AP 1:500) with COLO 320 cells lysate 1720ug. This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11742-1-AP (same clone as 11742-1-PBS)

    IF Staining of HeLa using 11742-1-AP (same clone as 11742-1-PBS)

    Immunofluorescent analysis of HeLa cells using 11742-1-AP (SPRR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SPRR3 Polyclonal antibody, PBS Only

SPRR3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11742-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

11742-1-PBS targets SPRR3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 SPRR3 fusion protein Ag2337 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 small proline-rich protein 3
别名
计算分子量 19 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC017802
基因名称 SPRR3
Gene ID (NCBI) 6707
RRIDAB_2195272
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBC9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPRR3, also named as Esophagin, Cornifin beta and SPRC, belongs to the cornifin (SPRR) family. It is cross-linked envelope protein of keratinocytes. Increased expression of SPRR3 appears to be involved in colorectal tumorigenesis. SPRR3 is a marker for terminal squamous cell differentiation.a potential novel therapeutic target for less advanced stages of Breast Cancer.