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  • Featured Product
  • KD/KO Validated

SPRR3 Polyclonal antibody, PBS Only

SPRR3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11742-1-PBS

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Synonyms

验证数据展示

  • WB analysis of HeLa using 11742-1-AP (same clone as 11742-1-PBS)

    WB analysis of HeLa using 11742-1-AP (same clone as 11742-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11742-1-AP (SPRR3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11742-1-AP (same clone as 11742-1-PBS)

    IHC staining of human colon cancer using 11742-1-AP (same clone as 11742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11742-1-AP (same clone as 11742-1-PBS)

    IHC staining of human colon cancer using 11742-1-AP (same clone as 11742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • IP experiment of COLO 320 using 11742-1-AP (same clone as 11742-1-PBS)

    IP experiment of COLO 320 using 11742-1-AP (same clone as 11742-1-PBS)

    IP result of anti-SPRR3 (IP:11742-1-AP, 3ug; Detection:11742-1-AP 1:500) with COLO 320 cells lysate 1720ug. This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11742-1-AP (same clone as 11742-1-PBS)

    IF Staining of HeLa using 11742-1-AP (same clone as 11742-1-PBS)

    Immunofluorescent analysis of HeLa cells using 11742-1-AP (SPRR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

产品信息

11742-1-PBS targets SPRR3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen SPRR3 fusion protein Ag2337 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name small proline-rich protein 3
Synonyms
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC017802
Gene Symbol SPRR3
Gene ID (NCBI) 6707
RRIDAB_2195272
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBC9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPRR3, also named as Esophagin, Cornifin beta and SPRC, belongs to the cornifin (SPRR) family. It is cross-linked envelope protein of keratinocytes. Increased expression of SPRR3 appears to be involved in colorectal tumorigenesis. SPRR3 is a marker for terminal squamous cell differentiation.a potential novel therapeutic target for less advanced stages of Breast Cancer.