验证数据展示
经过测试的应用
| Positive WB detected in | SW 1990 cells, Jurkat cells, A375 cells, Neuro-2a cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11383-1-AP targets SPRY2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SPRY2 fusion protein Ag1918 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sprouty homolog 2 (Drosophila) |
| 别名 | Spry-2, Spry 2, sprouty homolog 2 (Drosophila), Protein sprouty homolog 2, hSPRY2 |
| 计算分子量 | 35 kDa |
| 观测分子量 | 35-40 kDa, 60~65 kDa |
| GenBank蛋白编号 | BC015745 |
| 基因名称 | SPRY2 |
| Gene ID (NCBI) | 10253 |
| RRID | AB_2195287 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43597 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sprouty(SPRY) genes encode intracellular inhibitors of receptor tyrosine kinase (RTK) signaling pathways, including those triggered by fibroblast growth factors (Fgfs). In addition to its well-reported function as an inhibitor of the RAS/RAF/MEK signalling cascade, SPRY2 exerts its inhibitory role in the PI3K/AKT signalling pathway through PTEN, and inhibits ERK/MAPK activity via EGFR trafficking. SPRY2 was detected 60-65 kDa (PMID:29291435).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPRY2 antibody 11383-1-AP | Download protocol |
| IHC protocol for SPRY2 antibody 11383-1-AP | Download protocol |
| IF protocol for SPRY2 antibody 11383-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Crohns Colitis MSCs-exosomes can promote macrophage M2 polarization via exosomal miR-21-5p through Mesenteric injection: a promising way to attenuate murine colitis | ||
Int J Mol Sci Kub3 Deficiency Causes Aberrant Late Embryonic Lung Development in Mice by the FGF Signaling Pathway. | ||
J Cell Mol Med Exosomal miR-27 negatively regulates ROS production and promotes granulosa cells apoptosis by targeting SPRY2 in OHSS.
| ||
Toxicol Appl Pharmacol Hexachlorophene, a selective SHP2 inhibitor, suppresses proliferation and metastasis of KRAS-mutant NSCLC cells by inhibiting RAS/MEK/ERK and PI3K/AKT signaling pathways. | ||
Cell Biol Int Sprouty2 is involved in the control of osteoblast proliferation and differentiation through the FGF and BMP signaling pathways. | ||
Front Immunol Activated hepatic stellate cell-derived small extracellular vesicles facilitate M2 macrophage polarization and hepatoma progression via miR-27a-3p |