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SPRYD5 Polyclonal antibody

SPRYD5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20802-1-AP

Print datasheet

Synonyms

SPRY domain containing 5, SPRYD5, TRIM51

验证数据展示

  • WB analysis of Jurkat using 20802-1-AP

    WB analysis of Jurkat using 20802-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20802-1-AP (SPRYD5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 20802-1-AP

    IHC staining of human placenta using 20802-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20802-1-AP (SPRYD5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 20802-1-AP

    IHC staining of human placenta using 20802-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20802-1-AP (SPRYD5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, rat brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20802-1-AP targets SPRYD5 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, rat
Immunogen SPRYD5 fusion protein Ag14723 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SPRY domain containing 5
Synonyms SPRY domain containing 5, SPRYD5, TRIM51
Calculated Molecular Weight 293 aa, 34 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC005014
Gene Symbol SPRYD5
Gene ID (NCBI) 84767
RRIDAB_2878743
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSJ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPRYD5 antibody 20802-1-APDownload protocol
IHC protocol for SPRYD5 antibody 20802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols