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验证数据展示

  • WB analysis of Jurkat using 20802-1-AP

    WB analysis of Jurkat using 20802-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20802-1-AP (SPRYD5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 20802-1-AP

    IHC staining of human placenta using 20802-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20802-1-AP (SPRYD5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 20802-1-AP

    IHC staining of human placenta using 20802-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20802-1-AP (SPRYD5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SPRYD5 Polyclonal antibody

SPRYD5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20802-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

SPRY domain containing 5, SPRYD5, TRIM51

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, rat brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20802-1-AP targets SPRYD5 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原 SPRYD5 fusion protein Ag14723 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SPRY domain containing 5
别名 SPRY domain containing 5, SPRYD5, TRIM51
计算分子量 293 aa, 34 kDa
观测分子量 52 kDa
GenBank蛋白编号BC005014
基因名称 SPRYD5
Gene ID (NCBI) 84767
RRIDAB_2878743
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BSJ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPRYD5 antibody 20802-1-APDownload protocol
IHC protocol for SPRYD5 antibody 20802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols