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验证数据展示

  • WB analysis using 55227-1-AP

    WB analysis using 55227-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55227-1-AP (SPTA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse lung using 55227-1-AP

    IF Staining of mouse lung using 55227-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using SPTA1 antibody (55227-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of K-562 using 55227-1-AP

    FC experiment of K-562 using 55227-1-AP

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug SPTA1 Polyclonal antibody (55227-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

SPTA1 Polyclonal antibody

SPTA1 Polyclonal Antibody for WB, IF-P, FC (Intra), ELISA
Cat No. 55227-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF-P, FC (Intra), ELISA

别名

EL2, Alpha I spectrin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat red blood cells, human blood
Positive IF-P detected inmouse lung tissue
Positive FC (Intra) detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55227-1-AP targets SPTA1 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spectrin, alpha, erythrocytic 1 (elliptocytosis 2)
别名 EL2, Alpha I spectrin
计算分子量 280 kDa
观测分子量 280 kDa
GenBank蛋白编号NM_003126
基因名称 SPTA1
Gene ID (NCBI) 6708
RRIDAB_3086436
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02549
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha 1 Spectrin also known as SPTA1, EL2, belongs to the spectrin family. Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Mutations in SPTA1 result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3(PMID: 1541680, 8941647).

实验方案

Product Specific Protocols
WB protocol for SPTA1 antibody 55227-1-APDownload protocol
IF protocol for SPTA1 antibody 55227-1-APDownload protocol
FC protocol for SPTA1 antibody 55227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols