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SPTB Polyclonal antibody

SPTB Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26936-1-AP

Print datasheet

Synonyms

Spectrin beta chain, erythrocytic, Beta-I spectrin, Beta I spectrin

验证数据展示

  • WB analysis using 26936-1-AP

    WB analysis using 26936-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26936-1-AP (SPTB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 26936-1-AP

    IF Staining of U2OS using 26936-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using SPTB antibody (26936-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, mouse heart tissue
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy SPTB Ready-To-Use IHC Kit. SPTB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26936-1-AP targets SPTB in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SPTB fusion protein Ag25614 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name spectrin, beta, erythrocytic
Synonyms Spectrin beta chain, erythrocytic, Beta-I spectrin, Beta I spectrin
Observed Molecular Weight 246 kDa
GenBank Accession NumberBC136284
Gene Symbol SPTB
Gene ID (NCBI) 6710
RRIDAB_3669575
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11277
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta I spectrin also known as SPTB, SPTB1, belongs to the spectrin family. Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Mutations in SPTB have been associated with Elliptocytosis 3, spherocytosis type 2, and neonatal hemolytic anemia(PMID: 8018926, 8102379, 9005995).

实验方案

Product Specific Protocols
WB protocol for SPTB antibody 26936-1-APDownload protocol
IF protocol for SPTB antibody 26936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols