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验证数据展示

  • WB analysis using 26936-1-AP

    WB analysis using 26936-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26936-1-AP (SPTB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 26936-1-AP

    IF Staining of U2OS using 26936-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using SPTB antibody (26936-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SPTB Polyclonal antibody

SPTB Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26936-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Spectrin beta chain, erythrocytic, Beta-I spectrin, Beta I spectrin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, mouse heart tissue
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy SPTB Ready-To-Use IHC Kit. SPTB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26936-1-AP targets SPTB in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SPTB fusion protein Ag25614 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spectrin, beta, erythrocytic
别名 Spectrin beta chain, erythrocytic, Beta-I spectrin, Beta I spectrin
观测分子量 246 kDa
GenBank蛋白编号BC136284
基因名称 SPTB
Gene ID (NCBI) 6710
RRIDAB_3669575
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11277
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta I spectrin also known as SPTB, SPTB1, belongs to the spectrin family. Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Mutations in SPTB have been associated with Elliptocytosis 3, spherocytosis type 2, and neonatal hemolytic anemia(PMID: 8018926, 8102379, 9005995).

实验方案

Product Specific Protocols
WB protocol for SPTB antibody 26936-1-APDownload protocol
IF protocol for SPTB antibody 26936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols