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验证数据展示

  • WB analysis of HEK-293 using 25681-1-AP

    WB analysis of HEK-293 using 25681-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25681-1-AP (SPTBN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 25681-1-AP

    IF Staining of HEK-293 using 25681-1-AP

    Immunofluorescent analysis of HEK-293 cells using 25681-1-AP (SPTBN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 25681-1-AP

    WB analysis using 25681-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25681-1-AP (SPTBN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

SPTBN1 Polyclonal antibody

SPTBN1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25681-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Beta II spectrin, betaSpII, ELF, Fodrin beta chain, Spectrin beta chain, brain 1, SPTB2, SPTBN1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, mouse heart tissue, HEK-293 cells, SH-SY5Y cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy SPTBN1 Ready-To-Use IHC Kit. SPTBN1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25681-1-AP targets SPTBN1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SPTBN1 fusion protein Ag22459 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spectrin, beta, non-erythrocytic 1
别名 Beta II spectrin, betaSpII, ELF, Fodrin beta chain, Spectrin beta chain, brain 1, SPTB2, SPTBN1
计算分子量 2364 aa, 275 kDa
观测分子量 275 kDa
GenBank蛋白编号BC137282
基因名称 SPTBN1
Gene ID (NCBI) 6711
RRIDAB_2880191
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01082
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPTBN1 is the non-erythrocytic form of β-spectrin or β-fodrin. Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. Reduced expression of SPTBN1 has been found to correlate with shorter survival of patients with hepatocellular cancer, pancreatic cancer and other malignancies of the gastrointestinal tract, suggesting tumor suppression ability of this protein.

实验方案

Product Specific Protocols
WB protocol for SPTBN1 antibody 25681-1-APDownload protocol
IF protocol for SPTBN1 antibody 25681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols