验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human brain tissue, mouse brain, rat brain |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55107-1-AP targets SPTBN2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, drosophila |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spectrin, beta, non-erythrocytic 2 |
| 别名 | Spinocerebellar ataxia 5 protein, Spectrin beta-3, Spectrin beta chain, non-erythrocytic 2, Beta-III spectrin, Beta III spectrin |
| 计算分子量 | 271 kDa |
| 观测分子量 | 270 kDa |
| GenBank蛋白编号 | NM_006946 |
| 基因名称 | SPTBN2 |
| Gene ID (NCBI) | 6712 |
| RRID | AB_10804779 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15020 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPTBN2, also named as KIAA0302, belongs to the spectrin family. It probably plays an important role in neuronal membrane skeleton. Defects in SPTBN2 are the cause of spinocerebellar ataxia type 5 (SCA5). The antibody is specific to SPTBN2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPTBN2 antibody 55107-1-AP | Download protocol |
| IHC protocol for SPTBN2 antibody 55107-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem β-Spectrin regulates the Hippo signaling pathway and modulates the basal actin network.
| ||
Front Mol Neurosci Analyzing the mechanisms that facilitate the subtype-specific assembly of γ-aminobutyric acid type A receptors | ||
Cerebellum Nitric Oxide Critically Regulates Purkinje Neuron Dendritic Development Through a Metabotropic Glutamate Receptor Type 1-Mediated Mechanism. | ||
Dis Markers SPTBN2 Promotes the Progression of Thyroid Cancer by Accelerating G1/S Transition and Inhibiting Apoptosis | ||
Exp Ther Med Decreased SPTBN2 expression regulated by the ceRNA network is associated with poor prognosis and immune infiltration in low‑grade glioma |
