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验证数据展示

  • WB analysis using 31713-1-AP

    WB analysis using 31713-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31713-1-AP (SPTBN4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 31713-1-AP

    IF Staining of SH-SY5Y using 31713-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SPTBN4 antibody (31713-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

SPTBN4 Polyclonal antibody

SPTBN4 Polyclonal Antibody for WB, ELISA
Cat No. 31713-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

SPTBN3, KIAA1642, Beta IV spectrin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31713-1-AP targets SPTBN4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SPTBN4 fusion protein Ag36430 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spectrin, beta, non-erythrocytic 4
别名 SPTBN3, KIAA1642, Beta IV spectrin
计算分子量288kDa
观测分子量 250, 140 kDa
GenBank蛋白编号NM_020971
基因名称 SPTBN4
Gene ID (NCBI) 57731
RRIDAB_3670092
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9H254
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPTBN4 also known as beta-IV spectrin, encodes a nonerythrocytic member of the beta-spectrin protein family that is expressed in the brain, peripheral nervous system, pancreas, and skeletal muscle. Spectrins are rod-shaped proteins that were originally identified as part of the lattice-like cytoskeleton under the erythrocyte membrane(PMID: 11086001).

实验方案

Product Specific Protocols
WB protocol for SPTBN4 antibody 31713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols