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SPTLC1 Monoclonal antibody

SPTLC1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D11D7

Cat no : 66899-1-Ig

Print datasheet

Synonyms

Serine-palmitoyl-CoA transferase 1, LCB1, LCB 1, HSAN1, EC:2.3.1.50

验证数据展示

  • WB analysis using 66899-1-Ig

    WB analysis using 66899-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66899-1-Ig (SPTLC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 66899-1-Ig

    IHC staining of human colon cancer using 66899-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66899-1-Ig (SPTLC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 66899-1-Ig

    IHC staining of human colon cancer using 66899-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66899-1-Ig (SPTLC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HEK-293 using 66899-1-Ig

    WB analysis of HEK-293 using 66899-1-Ig

    WB result of SPTLC1 antibody (66899-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC1 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, RAW 264.7 cells, HT-29 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66899-1-Ig targets SPTLC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SPTLC1 fusion protein Ag7876 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serine palmitoyltransferase, long chain base subunit 1
Synonyms Serine-palmitoyl-CoA transferase 1, LCB1, LCB 1, HSAN1, EC:2.3.1.50
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC007085
Gene Symbol SPTLC1
Gene ID (NCBI) 10558
RRIDAB_2882228
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15269
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPTLC1 is a subunit of serine palmitoyltransferase (SPT) which is the key enzyme in sphingolipid biosynthesis and is essential for embryogenesis and cell survival. Mutations in the SPTLC1 gene (C133W, C133Y, V144D, and G387A) were reported to be responsible for the development of an inherited sensory neuropathy (hereditary sensory neuropathy type I, HSN1).

实验方案

Product Specific Protocols
WB protocol for SPTLC1 antibody 66899-1-IgDownload protocol
IHC protocol for SPTLC1 antibody 66899-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

EWS-FLI1 regulates and cooperates with core regulatory circuitry in Ewing sarcoma.

Authors - Xianping Shi
View Article