验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, mouse brain tissue, HEK-293 cells, HT-29 cells, HepG2 cells, NIH/3T3 cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
51012-2-AP targets SPTLC2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serine palmitoyltransferase, long chain base subunit 2 |
| 别名 | KIAA0526, LCB 2, LCB2, LCB2a, Serine palmitoyltransferase 2, SPT 2, SPT2, SPTLC2 |
| 计算分子量 | 63 kDa |
| 观测分子量 | 55-65 kDa |
| GenBank蛋白编号 | BC005123 |
| 基因名称 | SPTLC2 |
| Gene ID (NCBI) | 9517 |
| RRID | AB_2195870 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15270 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPTLC2 antibody 51012-2-AP | Download protocol |
| IHC protocol for SPTLC2 antibody 51012-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gastroenterology IRE1A Stimulates Hepatocyte-Derived Extracellular Vesicles That Promote Inflammation in Mice With Steatohepatitis. | ||
Nat Microbiol Sphingolipid biosynthesis induces a conformational change in the murine norovirus receptor and facilitates viral infection. | ||
Mol Cell Proteomics Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome. | ||
J Lipid Res ORMDL MISLOCALIZATION BY IMPAIRED AUTOPHAGY IN NIEMANN-PICK TYPE C DISEASE LEADS TO INCREASED DE NOVO SPHINGOLIPID BIOSYNTHESIS | ||
Cell Death Dis Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function.
| ||
J Biol Chem Liver-specific deficiency of serine palmitoyltransferase subunit 2 decreases plasma sphingomyelin and increases apolipoprotein E levels. |
