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验证数据展示

  • WB analysis using 17256-1-AP (same clone as 17256-1-PBS)

    WB analysis using 17256-1-AP (same clone as 17256-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 17256-1-AP (same clone as 17256-1-PBS)

    IP experiment of HeLa using 17256-1-AP (same clone as 17256-1-PBS)

    IP result of anti-SQRDL (IP:17256-1-AP, 4ug; Detection:17256-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

  • WB analysis using 17256-1-AP (same clone as 17256-1-PBS)

    WB analysis using 17256-1-AP (same clone as 17256-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 17256-1-AP (same clone as 17256-1-PBS)

    IHC staining of mouse liver using 17256-1-AP (same clone as 17256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SQRDL antibody included
  • IHC staining of rat liver using 17256-1-AP (same clone as 17256-1-PBS)

    IHC staining of rat liver using 17256-1-AP (same clone as 17256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SQRDL antibody included
  • IHC staining of mouse brain using 17256-1-AP (same clone as 17256-1-PBS)

    IHC staining of mouse brain using 17256-1-AP (same clone as 17256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SQRDL antibody included
  • Featured Product
  • KD/KO验证

SQRDL Polyclonal antibody, PBS Only

SQRDL Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17256-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

CGI 44, CGI-44, EC:1.8.5.8, SQOR, Sulfide dehydrogenase-like

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SQRDL Ready-To-Use IHC Kit. SQRDL primary antibody included.

产品信息

17256-1-PBS targets SQRDL in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10762

Product name: Recombinant human SQRDL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 100-450 aa of BC016836

Sequence: GRPTASVIPSGVEWIKARVTELNPDKNCIHTDDDEKISYRYLIIALGIQLDYEKIKGLPEGFAHPKIGSNYSVKTVEKTWKALQDFKEGNAIFTFPNTPVKCAGAPQKIMYLSEAYFRKTGKRSKANIIFNTSLGAIFGVKKYADALQEIIQERNLTVNYKKNLIEVRADKQEAVFENLDKPGETQVISYEMLHVTPPMSPPDVLKTSPVADAAGWVDVDKETLQHRRYPNVFGIGDCTNLPTSKTAAAVAAQSGILDRTISVIMKNQTPTKKYDGYTSCPLVTGYNRVILAEFDYKAEPLETFPFDQSKERLSMYLMKADLMPFLYWNMMLRGYWGGPAFLRKLFHLGMS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sulfide quinone reductase-like (yeast)
别名 CGI 44, CGI-44, EC:1.8.5.8, SQOR, Sulfide dehydrogenase-like
计算分子量 450 aa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC016836
基因名称 SQRDL
Gene ID (NCBI) 58472
RRIDAB_2195894
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6N5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SQRDL(Sulfide:quinone oxidoreductase, mitochondrial) catalyzes the oxidation of hydrogen sulfide, with the help of a quinone.Although the SQRDL precursor protein displays a molecular weight of 50 kDa,a prominent band at about 46 kDa is detected by western blot. This discrepancy reflects the cleavage of an N-terminal targeting sequence of 4 kDa during the import of the protein into the mitochondria(PMID:22067608).SQRDL is one of the enzymes involved in H2S signaling in a discrete population of neurons, oligodendrocytes, and endothelial cells.

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