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SQRDL Monoclonal antibody

SQRDL Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1B4E3

Cat no : 68459-1-Ig

Print datasheet

Synonyms

Sulfide dehydrogenase-like, SQOR, EC:1.8.5.8, CGI-44, CGI 44

验证数据展示

  • WB analysis using 68459-1-Ig

    WB analysis using 68459-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68459-1-Ig (SQRDL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 68459-1-Ig

    WB analysis of A549 using 68459-1-Ig

    WB result of SQRDL antibody (68459-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SQRDL transfected A549 cells.

  • IP experiment of HeLa using 68459-1-Ig

    IP experiment of HeLa using 68459-1-Ig

    IP result of anti-SQRDL (IP:68459-1-Ig, 4ug; Detection:68459-1-Ig 1:3000) with HeLa cells lysate 1560 ug.

  • IHC staining of human skin cancer using 68459-1-Ig

    IHC staining of human skin cancer using 68459-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 68459-1-Ig (SQRDL antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SQRDL antibody included
  • IF Staining of HepG2 using 68459-1-Ig

    IF Staining of HepG2 using 68459-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SQRDL antibody (68459-1-Ig, Clone: 1B4E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, HT-29 cells, hTERT-RPE1 cells, THP-1 cells, pig liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SQRDL Ready-To-Use IHC Kit. SQRDL primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68459-1-Ig targets SQRDL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, pig
Immunogen SQRDL fusion protein Ag10762 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name sulfide quinone reductase-like (yeast)
Synonyms Sulfide dehydrogenase-like, SQOR, EC:1.8.5.8, CGI-44, CGI 44
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC016836
Gene Symbol SQRDL
Gene ID (NCBI) 58472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6N5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SQRDL(Sulfide:quinone oxidoreductase, mitochondrial) catalyzes the oxidation of hydrogen sulfide, with the help of a quinone.Although the SQRDL precursor protein displays a molecular weight of 50 kDa,a prominent band at about 46 kDa is detected by western blot. This discrepancy reflects the cleavage of an N-terminal targeting sequence of 4 kDa during the import of the protein into the mitochondria(PMID:22067608).SQRDL is one of the enzymes involved in H2S signaling in a discrete population of neurons, oligodendrocytes, and endothelial cells.

实验方案

Product Specific Protocols
WB protocol for SQRDL antibody 68459-1-IgDownload protocol
IHC protocol for SQRDL antibody 68459-1-IgDownload protocol
IF protocol for SQRDL antibody 68459-1-IgDownload protocol
IP protocol for SQRDL antibody 68459-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols