• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • Cytometric bead array standard curve of MP00097-1

    Cytometric bead array standard curve of MP00097-1

    Cytometric bead array standard curve of MP00097-1, SQS/FDFT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83020-2-PBS. Detection antibody: 83020-4-PBS. Standard: Ag3743. Range: 0.625-20 ng/mL.

  • Sandwich ELISA standard curve of MP00097-1

    Sandwich ELISA standard curve of MP00097-1

    Sandwich ELISA standard curve of MP00097-1, Human FDFT1 Recombinant Matched Antibody Pair - PBS only. 83020-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3743. 83020-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP00097-1

    Sample test of MP00097-1

    Serum of eight individual healthy human donors was measured. The FDFT1 concentration of detected samples was determined to be 1,817 pg/mL with a range of 368 - 5,103 pg/mL.

  • Sample test of MP00097-1

    Sample test of MP00097-1

    The mean FDFT1 concentration was determined to be 8.7 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

  • IF Staining of HepG2 using 83020-2-RR (same clone as 83020-2-PBS)

    IF Staining of HepG2 using 83020-2-RR (same clone as 83020-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDFT1 antibody (83020-2-RR, Clone: 230362F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83020-2-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83020-2-RR (same clone as 83020-2-PBS)

    FC experiment of U2OS using 83020-2-RR (same clone as 83020-2-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug FDFT1 Recombinant antibody (83020-2-RR, Clone:230362F3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83020-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00097-3

    Cytometric bead array standard curve of MP00097-3

    Cytometric bead array standard curve of MP00097-3, SQS/FDFT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83020-3-PBS. Detection antibody: 83020-2-PBS. Standard: Ag3743. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83020-2-PBS

    Affinity and Kinetic Characterization of 83020-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83020-2-PBS against Human FDFT1 was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 83020-2-RR

    Affinity and Kinetic Characterization of 83020-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83020-2-RR against Human FDFT1 were performed. The affinity constant is 0.107 nM.

FDFT1 Recombinant antibody, PBS Only (Capture/Detector)

FDFT1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83020-2-PBS

产品说明书

CloneNo. 230362F3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Farnesyl-diphosphate farnesyltransferase 1, Farnesyl-diphosphate farnesyltransferase, ERG9, EC:2.5.1.21, DGPT

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

83020-2-PBS targets FDFT1 as part of a matched antibody pair:

MP00097-1: 83020-2-PBS capture and 83020-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00097-3: 83020-3-PBS capture and 83020-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 FDFT1 fusion protein Ag3743 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 farnesyl-diphosphate farnesyltransferase 1
别名 Farnesyl-diphosphate farnesyltransferase 1, Farnesyl-diphosphate farnesyltransferase, ERG9, EC:2.5.1.21, DGPT
计算分子量 417 aa, 48 kDa
GenBank蛋白编号BC029641
基因名称 FDFT1
Gene ID (NCBI) 2222
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP37268
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C