验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MCF-7 cells, HepG2 cells |
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | human breast cancer tissue, mouse brain tissue, mouse kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, U2OS cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55274-1-AP targets P62/SQSTM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IF, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | sequestosome 1 |
| Synonyms | P62, P62/SQSTM1, sequestosome 1, SQSTM1, Ubiquitin binding protein p62 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | NM_003900 |
| Gene Symbol | P62/SQSTM1 |
| Gene ID (NCBI) | 8878 |
| RRID | AB_11182278 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13501 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SQSTM1, also named as ORCA, OSIL, p60, EBIAP, A170, PDB3, ZIP3, p62 and p62B, is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. It may play a role in titin/TTN downstream signaling in muscle cells. SQSTM1 may regulate signaling cascades through ubiquitination. It is an adapter that mediates the interaction between TRAF6 and CYLD. SQSTM1 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels (PMID: 10356400). This antibody is specific to SQSTM1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for P62/SQSTM1 antibody 55274-1-AP | Download protocol |
| IHC protocol for P62/SQSTM1 antibody 55274-1-AP | Download protocol |
| IF protocol for P62/SQSTM1 antibody 55274-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Autophagy-mediated apoptosis eliminates aneuploid cells in a mouse model of chromosome mosaicism. | ||
Cell Death Differ CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation. | ||
Autophagy An integrative multi-omics approach uncovers the regulatory role of CDK7 and CDK4 in autophagy activation induced by silica nanoparticles. | ||
Front Pharmacol Morroniside Inhibits H2O2-Induced Podocyte Apoptosis by Down-Regulating NOX4 Expression Controlled by Autophagy In Vitro. | ||
Front Pharmacol Resolvin D1 attenuates CCl4 Induced Liver Fibrosis by Inhibiting Autophagy-Mediated HSC activation via AKT/mTOR Pathway | ||
Nutrients Punicalagin Prevents Inflammation in LPS-Induced RAW264.7 Macrophages by Inhibiting FoxO3a/Autophagy Signaling Pathway. |
