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P62/SQSTM1 Polyclonal antibody

P62/SQSTM1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Cat no : 55274-1-AP

Print datasheet

Synonyms

P62, P62/SQSTM1, sequestosome 1, SQSTM1, Ubiquitin binding protein p62

验证数据展示

WB analysis using 55274-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55274-1-AP (P62/SQSTM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 55274-1-AP
WB result of P62/SQSTM1 antibody (55274-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62/SQSTM1 transfected HEK-293 cells.
IP experiment of U2OS using 55274-1-AP
U2OS lysates prepared and IP of SQSTM1 performed using 1.0 μg of 55274-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IF Staining of U2OS using 55274-1-AP
U2OS WT cells (yellow outline) and SQSTM1 KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 55274-1-AP at 1:300. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IF Staining of HepG2 using 55274-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 55274-1-AP (P62/SQSTM1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IHC staining of human breast cancer using 55274-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 55274-1-AP (SQSTM1 antibody) at dilution of 1:50 (under 40x lens).
Ready-to-use IHC kits available with P62,SQSTM1 antibody included
IHC staining of human breast cancer using 55274-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 55274-1-AP (SQSTM1 antibody) at dilution of 1:50 (under 10x lens).
Ready-to-use IHC kits available with P62,SQSTM1 antibody included

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Cat No. 55274-1-AP

Size

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经过测试的应用

Positive WB detected in	HEK-293 cells, MCF-7 cells, HepG2 cells
Positive IP detected in	U2OS cells
Positive IHC detected in	human breast cancer tissue, mouse brain tissue, mouse kidney tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, U2OS cells

Planning an IHC experiment? We recommend our IHCeasy P62,SQSTM1 Ready-To-Use IHC Kit. P62,SQSTM1 primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 22 publications below
IHC	See 3 publications below
IF	See 5 publications below
IP	See 1 publications below

产品信息

55274-1-AP targets P62/SQSTM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IP, IF, IHC, ELISA Application Description
Cited Applications	WB, IHC, IF, IP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	Peptide 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	sequestosome 1
Synonyms	P62, P62/SQSTM1, sequestosome 1, SQSTM1, Ubiquitin binding protein p62
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	60 kDa
GenBank Accession Number	NM_003900
Gene Symbol	P62/SQSTM1
Gene ID (NCBI)	8878
RRID	AB_11182278
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13501
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

SQSTM1, also named as ORCA, OSIL, p60, EBIAP, A170, PDB3, ZIP3, p62 and p62B, is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. It may play a role in titin/TTN downstream signaling in muscle cells. SQSTM1 may regulate signaling cascades through ubiquitination. It is an adapter that mediates the interaction between TRAF6 and CYLD. SQSTM1 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels (PMID: 10356400). This antibody is specific to SQSTM1.

实验方案

Product Specific Protocols
WB protocol for P62/SQSTM1 antibody 55274-1-AP	Download protocol
IHC protocol for P62/SQSTM1 antibody 55274-1-AP	Download protocol
IF protocol for P62/SQSTM1 antibody 55274-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IF	Nat Commun Autophagy-mediated apoptosis eliminates aneuploid cells in a mouse model of chromosome mosaicism. Authors - Shruti Singla View Article
human	WB	Cell Death Differ CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation. Authors - Yina Liao View Article
human	WB	Autophagy An integrative multi-omics approach uncovers the regulatory role of CDK7 and CDK4 in autophagy activation induced by silica nanoparticles. Authors - Chen Ruan View Article
mouse	WB,IF	Front Pharmacol Morroniside Inhibits H2O2-Induced Podocyte Apoptosis by Down-Regulating NOX4 Expression Controlled by Autophagy In Vitro. Authors - Xue Gao View Article
human,mouse	WB	Front Pharmacol Resolvin D1 attenuates CCl4 Induced Liver Fibrosis by Inhibiting Autophagy-Mediated HSC activation via AKT/mTOR Pathway Authors - Jiahuan Li View Article
mouse	WB	Nutrients Punicalagin Prevents Inflammation in LPS-Induced RAW264.7 Macrophages by Inhibiting FoxO3a/Autophagy Signaling Pathway. Authors - Yuan Cao View Article

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P62/SQSTM1 Polyclonal antibody

P62/SQSTM1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

验证数据展示

WB analysis using 55274-1-AP

WB analysis of HEK-293 using 55274-1-AP

IP experiment of U2OS using 55274-1-AP

IF Staining of U2OS using 55274-1-AP

IF Staining of HepG2 using 55274-1-AP

IHC staining of human breast cancer using 55274-1-AP

IHC staining of human breast cancer using 55274-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Autophagy-mediated apoptosis eliminates aneuploid cells in a mouse model of chromosome mosaicism.

CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.

An integrative multi-omics approach uncovers the regulatory role of CDK7 and CDK4 in autophagy activation induced by silica nanoparticles.

Morroniside Inhibits H2O2-Induced Podocyte Apoptosis by Down-Regulating NOX4 Expression Controlled by Autophagy In Vitro.

Resolvin D1 attenuates CCl4 Induced Liver Fibrosis by Inhibiting Autophagy-Mediated HSC activation via AKT/mTOR Pathway

Punicalagin Prevents Inflammation in LPS-Induced RAW264.7 Macrophages by Inhibiting FoxO3a/Autophagy Signaling Pathway.