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验证数据展示

  • WB analysis of A431 using 18699-1-AP

    WB analysis of A431 using 18699-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 18699-1-AP (SRA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 18699-1-AP

    IF Staining of A431 using 18699-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 18699-1-AP (SRA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SRA1 Polyclonal antibody

SRA1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18699-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, ELISA and More (1)

别名

pp7684, SRA, SRA1, SRAP, STRAA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, MCF-7 cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SRA1 Ready-To-Use IHC Kit. SRA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18699-1-AP targets SRA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 steroid receptor RNA activator 1
别名 pp7684, SRA, SRA1, SRAP, STRAA1
计算分子量 26 kDa
观测分子量 37 kDa
GenBank蛋白编号BC156760
基因名称 SRA1
Gene ID (NCBI) 10011
RRIDAB_2270887
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HD15
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Steroid receptor RNA activator 1 (SRA1), also known as seroid receptor RNA activator protein (SRAP) or SRA, is involved in modulating the activity of multiple transcription factors including the estrogen receptor (ER) (PMID: 20398657). It may play a role in tumorigenesis (PMID: 16152589). The SRA1 gene encodes both SRA1 protein and a non-coding functional RNA that functions as part of a ribonucleoprotein complex activating steroid receptor induced transcription.

实验方案

Product Specific Protocols
WB protocol for SRA1 antibody 18699-1-APDownload protocol
IF protocol for SRA1 antibody 18699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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