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验证数据展示

  • IP experiment of mouse brain using 24931-1-AP

    IP experiment of mouse brain using 24931-1-AP

    IP result of anti-SRBD1 (IP:24931-1-AP, 4ug; Detection:24931-1-AP 1:300) with mouse brain tissue lysate 4000ug.

  • IHC staining of human brain using 24931-1-AP

    IHC staining of human brain using 24931-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24931-1-AP (SRBD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 24931-1-AP

    IHC staining of human brain using 24931-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24931-1-AP (SRBD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 24931-1-AP

    WB analysis using 24931-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24931-1-AP (SRBD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

SRBD1 Polyclonal antibody

SRBD1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 24931-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Synonyms

S1 RNA binding domain 1, SRBD1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24931-1-AP targets SRBD1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SRBD1 fusion protein Ag21661 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name S1 RNA binding domain 1
Synonyms S1 RNA binding domain 1, SRBD1
Calculated Molecular Weight 995 aa, 112 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC032538
Gene Symbol SRBD1
Gene ID (NCBI) 55133
RRIDAB_2879805
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N5C6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S1 RNA-binding domain-containing protein 1 (SRBD1) is a 995 amino acid protein, which contains one S1 motif domain. SRBD1 is a susceptibility gene for normal tension glaucoma (NTG) and may be involved in cell growth inhibition and apoptosis. Two isoforms of SRBD1 exist due to alternative splicing events, and the gene which encodes SRBD1 maps to human chromosome 2p21.

实验方案

Product Specific Protocols
WB protocol for SRBD1 antibody 24931-1-APDownload protocol
IHC protocol for SRBD1 antibody 24931-1-APDownload protocol
IP protocol for SRBD1 antibody 24931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols