验证数据展示
经过测试的应用
Positive WB detected in | SH-SY5Y cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
24931-1-AP targets SRBD1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | SRBD1 fusion protein Ag21661 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | S1 RNA binding domain 1 |
Synonyms | S1 RNA binding domain 1, SRBD1 |
Calculated Molecular Weight | 995 aa, 112 kDa |
Observed Molecular Weight | 100-120 kDa |
GenBank Accession Number | BC032538 |
Gene Symbol | SRBD1 |
Gene ID (NCBI) | 55133 |
RRID | AB_2879805 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8N5C6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
S1 RNA-binding domain-containing protein 1 (SRBD1) is a 995 amino acid protein, which contains one S1 motif domain. SRBD1 is a susceptibility gene for normal tension glaucoma (NTG) and may be involved in cell growth inhibition and apoptosis. Two isoforms of SRBD1 exist due to alternative splicing events, and the gene which encodes SRBD1 maps to human chromosome 2p21.
实验方案
Product Specific Protocols | |
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WB protocol for SRBD1 antibody 24931-1-AP | Download protocol |
IHC protocol for SRBD1 antibody 24931-1-AP | Download protocol |
IP protocol for SRBD1 antibody 24931-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |