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验证数据展示

  • WB analysis using 21952-1-AP

    WB analysis using 21952-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21952-1-AP (NCOA1/SRC-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

NCOA1/SRC-1 Polyclonal antibody

NCOA1/SRC-1 Polyclonal Antibody for WB, ELISA
Cat No. 21952-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

bHLHe42, bHLHe74, F SRC 1, KAT13A, NCoA 1, NCOA1, nuclear receptor coactivator 1, Protein Hin 2, RIP160, SRC 1, SRC1, Steroid receptor coactivator 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, Raji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21952-1-AP targets NCOA1/SRC-1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 NCOA1/SRC-1 fusion protein Ag16603 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear receptor coactivator 1
别名 bHLHe42, bHLHe74, F SRC 1, KAT13A, NCoA 1, NCOA1, nuclear receptor coactivator 1, Protein Hin 2, RIP160, SRC 1, SRC1, Steroid receptor coactivator 1
计算分子量 1441 aa, 157 kDa
观测分子量 140-160 kDa
GenBank蛋白编号BC111533
基因名称 SRC 1/NCOA1
Gene ID (NCBI) 8648
RRIDAB_2935455
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ15788
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear receptor coactivator 1, also named SRC-1, directly binds nuclear receptors and stimulates transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR, and ER), retinoids (RXRs), thyroid hormone (TRs), and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B, and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via the remodeling of chromatin, and possibly act by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.

实验方案

Product Specific Protocols
WB protocol for NCOA1/SRC-1 antibody 21952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols