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  • Featured Product
  • KD/KO Validated

SRD5A1 Monoclonal antibody, PBS Only

SRD5A1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D5D8

Cat no : 66329-1-PBS

Print datasheet

Synonyms

S5AR 1, SR type 1, SRD5A1, Steroid 5 alpha reductase 1

验证数据展示

  • WB analysis of human testis using 66329-1-Ig (same clone as 66329-1-PBS)

    WB analysis of human testis using 66329-1-Ig (same clone as 66329-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

    IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

    IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66329-1-Ig (same clone as 66329-1-PBS)

    IF Staining of HeLa using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66329-1-Ig(SRD5A1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • WB analysis using 66329-1-Ig (same clone as 66329-1-PBS)

    WB analysis using 66329-1-Ig (same clone as 66329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66329-1-Ig (SRD5A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • IF Staining of HEPG2 using 66329-1-Ig (same clone as 66329-1-PBS)

    IF Staining of HEPG2 using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEPG2 cells using SRD5A1 antibody (66329-1-Ig, Clone: 1D5D8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

产品信息

66329-1-PBS targets SRD5A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen SRD5A1 fusion protein Ag23351 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
Synonyms S5AR 1, SR type 1, SRD5A1, Steroid 5 alpha reductase 1
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC007033
Gene Symbol SRD5A1
Gene ID (NCBI) 6715
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18405
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Steroid 5-alpha-reductase (SRD5A1)   is a minor component of the reductase activity in prostate although the gene was originally cloned from prostate. On the other hand, SRD5A1 appears to be the predominant isozyme of steroid 5-alpha-reductase in the scalp and elsewhere in the skin.