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  • Featured Product
  • KD/KO Validated

SRD5A1 Monoclonal antibody, PBS Only

SRD5A1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1D5D8

Cat No : 66329-1-PBS

Print datasheet

Synonyms

S5AR 1, SR type 1, SRD5A1, Steroid 5 alpha reductase 1

验证数据展示

  • WB analysis of human testis using 66329-1-Ig (same clone as 66329-1-PBS)

    WB analysis of human testis using 66329-1-Ig (same clone as 66329-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

    IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

    IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66329-1-Ig (same clone as 66329-1-PBS)

    IF Staining of HeLa using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66329-1-Ig(SRD5A1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • WB analysis using 66329-1-Ig (same clone as 66329-1-PBS)

    WB analysis using 66329-1-Ig (same clone as 66329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66329-1-Ig (SRD5A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • IF Staining of HEPG2 using 66329-1-Ig (same clone as 66329-1-PBS)

    IF Staining of HEPG2 using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEPG2 cells using SRD5A1 antibody (66329-1-Ig, Clone: 1D5D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

产品信息

66329-1-PBS targets SRD5A1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human
Immunogen SRD5A1 fusion protein Ag23351 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
Synonyms S5AR 1, SR type 1, SRD5A1, Steroid 5 alpha reductase 1
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC007033
Gene Symbol SRD5A1
Gene ID (NCBI) 6715
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18405
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Steroid 5-alpha-reductase (SRD5A1)   is a minor component of the reductase activity in prostate although the gene was originally cloned from prostate. On the other hand, SRD5A1 appears to be the predominant isozyme of steroid 5-alpha-reductase in the scalp and elsewhere in the skin.