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  • Featured Product
  • KD/KO Validated

SREBF1 Monoclonal antibody, PBS Only

SREBF1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B6G5

Cat no : 66875-1-PBS

Print datasheet

Synonyms

SREBP 1, SREBF 1, Processed sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1, bHLHd1

验证数据展示

  • IHC staining of human kidney using 66875-1-Ig (same clone as 66875-1-PBS)

    IHC staining of human kidney using 66875-1-Ig (same clone as 66875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66875-1-Ig (SREBF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SREBF1 antibody included
  • IF Staining of HepG2 using 66875-1-Ig (same clone as 66875-1-PBS)

    IF Staining of HepG2 using 66875-1-Ig (same clone as 66875-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SREBF1 antibody (66875-1-Ig, Clone: 1B6G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). CL594-Phalloidin stains the F-actin (red). DAPI (blue). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66875-1-Ig (same clone as 66875-1-PBS)

    WB analysis of HeLa using 66875-1-Ig (same clone as 66875-1-PBS)

    WB result of SREBF1 antibody (66875-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SREBF1 transfected HeLa cells. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

    WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

    WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SREBF1 Ready-To-Use IHC Kit. SREBF1 primary antibody included.

产品信息

66875-1-PBS targets SREBF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen SREBF1 fusion protein Ag5484 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sterol regulatory element binding transcription factor 1
Synonyms SREBP 1, SREBF 1, Processed sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1, bHLHd1
Calculated Molecular Weight 1177 aa, 125 kDa
Observed Molecular Weight 125 kDa
GenBank Accession NumberBC063281
Gene Symbol SREBF1
Gene ID (NCBI) 6720
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP36956
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125kd precursor form and the 68kd mature form of human SREBF1.