• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SREBF1 Recombinant antibody, PBS Only

SREBF1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1P4

Cat no : 82684-3-PBS

Print datasheet

Synonyms

SREBP 1, SREBF 1, Processed sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1, bHLHd1

验证数据展示

  • WB analysis of HeLa using 82684-3-RR (same clone as 82684-3-PBS)

    WB analysis of HeLa using 82684-3-RR (same clone as 82684-3-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 82684-3-RR (SREBF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82684-3-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82684-3-RR (same clone as 82684-3-PBS)

    FC experiment of HepG2 using 82684-3-RR (same clone as 82684-3-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug SREBF1 Recombinant antibody (82684-3-RR, Clone:1P4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82684-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82684-3-RR

    Affinity and Kinetic Characterization of 82684-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82684-3-RR against Human SREBF1 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy SREBF1 Ready-To-Use IHC Kit. SREBF1 primary antibody included.

产品信息

82684-3-PBS targets SREBF1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen SREBF1 fusion protein Ag5219 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sterol regulatory element binding transcription factor 1
Synonyms SREBP 1, SREBF 1, Processed sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1, bHLHd1
Calculated Molecular Weight 1177 aa, 125 kDa
Observed Molecular Weight125 kDa
GenBank Accession NumberBC063281
Gene Symbol SREBF1
Gene ID (NCBI) 6720
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36956
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267].