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验证数据展示

  • WB analysis of HeLa using 82684-3-RR (same clone as 82684-3-PBS)

    WB analysis of HeLa using 82684-3-RR (same clone as 82684-3-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 82684-3-RR (SREBF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82684-3-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 82684-3-RR (same clone as 82684-3-PBS)

    WB analysis of MCF-7 using 82684-3-RR (same clone as 82684-3-PBS)

    WB result of SREBF1 antibody (82684-3-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SREBF1 transfected MCF-7 cells. This data was developed using the same antibody clone with 82684-3-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82684-3-RR (same clone as 82684-3-PBS)

    FC experiment of HepG2 using 82684-3-RR (same clone as 82684-3-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug SREBF1 Recombinant antibody (82684-3-RR, Clone:1P4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82684-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82684-3-RR

    Affinity and Kinetic Characterization of 82684-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82684-3-RR against Human SREBF1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

SREBF1 Recombinant antibody, PBS Only

SREBF1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82684-3-PBS

产品说明书

CloneNo. 1P4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

SREBP 1, SREBF 1, Processed sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1, bHLHd1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SREBF1 Ready-To-Use IHC Kit. SREBF1 primary antibody included.

产品信息

82684-3-PBS targets SREBF1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 SREBF1 fusion protein Ag5219 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sterol regulatory element binding transcription factor 1
别名 SREBP 1, SREBF 1, Processed sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1, bHLHd1
计算分子量 1177 aa, 125 kDa
观测分子量68 kDa, 125 kDa
GenBank蛋白编号BC063281
基因名称 SREBF1
Gene ID (NCBI) 6720
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36956
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267].