验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse kidney tissue, HepG2 cells, human kidney tissue, HEK-293 cells, rat kidney tissue, HeLa cells, rat liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue, human kidney tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SREBF2 Ready-To-Use IHC Kit. SREBF2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14508-1-AP targets SREBF2 in WB, IHC, IF, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, chIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken, hamster |
| 免疫原 | SREBF2 fusion protein Ag5967 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sterol regulatory element binding transcription factor 2 |
| 别名 | bHLHd2, SREBF2, SREBP 2, SREBP2 |
| 计算分子量 | 124 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC056158 |
| 基因名称 | SREBF2 |
| Gene ID (NCBI) | 6721 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q12772 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis. SREBF2 expression is dynamically regulated in response to nutritional and hormonal cues.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SREBF2 antibody 14508-1-AP | Download protocol |
| IHC protocol for SREBF2 antibody 14508-1-AP | Download protocol |
| IP protocol for SREBF2 antibody 14508-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1. | ||
Nature TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis. | ||
Environ Pollut Prenatal exposure to diesel exhaust PM2.5 programmed non-alcoholic fatty liver disease differently in adult male offspring of mice fed normal chow and a high-fat diet. | ||
Arterioscler Thromb Vasc Biol RP5-833A20.1/miR-382-5p/NFIA-Dependent Signal Transduction Pathway Contributes to the Regulation of Cholesterol Homeostasis and Inflammatory Reaction. | ||
J Lipid Res Intracellular hepatitis B virus increases hepatic cholesterol deposition in alcoholic fatty liver via hepatitis B core protein. |
