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  • Featured Product
  • KD/KO Validated

SRF Monoclonal antibody, PBS Only

SRF Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G8F4

Cat no : 66742-1-PBS

Print datasheet

Synonyms

Serum response factor, MCM1, 1G8F4

验证数据展示

  • WB analysis using 66742-1-Ig (same clone as 66742-1-PBS)

    WB analysis using 66742-1-Ig (same clone as 66742-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66742-1-Ig (same clone as 66742-1-PBS)

    WB analysis of MCF-7 using 66742-1-Ig (same clone as 66742-1-PBS)

    WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66742-1-Ig (same clone as 66742-1-PBS)

    IF Staining of HepG2 using 66742-1-Ig (same clone as 66742-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SRF antibody (66742-1-Ig, Clone: 1G8F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

  • WB analysis using 66742-1-Ig (same clone as 66742-1-PBS)

    WB analysis using 66742-1-Ig (same clone as 66742-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SRF Ready-To-Use IHC Kit. SRF primary antibody included.

产品信息

66742-1-PBS targets SRF in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SRF fusion protein Ag10386 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name serum response factor (c-fos serum response element-binding transcription factor)
Synonyms Serum response factor, MCM1, 1G8F4
Calculated Molecular Weight 508 aa, 52 kDa
GenBank Accession NumberBC048211
Gene Symbol SRF
Gene ID (NCBI) 6722
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11831
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).