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验证数据展示

  • WB analysis using 66742-1-Ig (same clone as 66742-1-PBS)

    WB analysis using 66742-1-Ig (same clone as 66742-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66742-1-Ig (same clone as 66742-1-PBS)

    WB analysis of MCF-7 using 66742-1-Ig (same clone as 66742-1-PBS)

    WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66742-1-Ig (same clone as 66742-1-PBS)

    IF Staining of HepG2 using 66742-1-Ig (same clone as 66742-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SRF antibody (66742-1-Ig, Clone: 1G8F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

  • WB analysis using 66742-1-Ig (same clone as 66742-1-PBS)

    WB analysis using 66742-1-Ig (same clone as 66742-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SRF Monoclonal antibody, PBS Only

SRF Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66742-1-PBS

产品说明书

CloneNo. 1G8F4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Serum response factor, MCM1, 1G8F4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SRF Ready-To-Use IHC Kit. SRF primary antibody included.

产品信息

66742-1-PBS targets SRF in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SRF fusion protein Ag10386 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 serum response factor (c-fos serum response element-binding transcription factor)
别名 Serum response factor, MCM1, 1G8F4
计算分子量 508 aa, 52 kDa
GenBank蛋白编号BC048211
基因名称 SRF
Gene ID (NCBI) 6722
RRIDAB_2882090
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11831
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).