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SRFBP1 Polyclonal antibody

SRFBP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12792-1-AP

Print datasheet

Synonyms

BUD22, P49, p49/STRAP, Rlb1, SRFBP1, STRAP

验证数据展示

  • IHC staining of human pancreas cancer using 12792-1-AP

    IHC staining of human pancreas cancer using 12792-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12792-1-AP (SRFBP1 antibody) at dilution of 1:100 (under 10x lens).

  • WB analysis of mouse heart using 12792-1-AP

    WB analysis of mouse heart using 12792-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12792-1-AP (SRFBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse heart tissue, HeLa cells, HepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12792-1-AP targets SRFBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SRFBP1 fusion protein Ag3517 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serum response factor binding protein 1
Synonyms BUD22, P49, p49/STRAP, Rlb1, SRFBP1, STRAP
Calculated Molecular Weight 429 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC031222
Gene Symbol SRFBP1
Gene ID (NCBI) 153443
RRIDAB_2877882
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NEF9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRFBP1, also named as P49, p49/STRAP and STRAP, is abundantly expressed in heart and skeletal muscle, and at much lower levels in brain and lung. It may be involved in regulating transcriptional activation of cardiac genes during the aging process. SRFBP1 may play a role in biosynthesis and/or processing of SLC2A4 in adipose cells. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human SRFBP1.

实验方案

Product Specific Protocols
WB protocol for SRFBP1 antibody 12792-1-APDownload protocol
IHC protocol for SRFBP1 antibody 12792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols