SRGAP1 Polyclonal antibody
SRGAP1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue, Apoptosised HeLa cells, mouse kidney tissue, HEK-293 cells, SH-SY5Y cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human liver cancer tissue, mouse testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SRGAP1 Ready-To-Use IHC Kit. SRGAP1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13252-1-AP targets SRGAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IP, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | SRGAP1 fusion protein Ag4104 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | SLIT-ROBO Rho GTPase activating protein 1 |
| Synonyms | ARHGAP13, KIAA1304, SRGAP1 |
| Calculated Molecular Weight | 1085 aa, 124 kDa |
| Observed Molecular Weight | 124 kDa |
| GenBank Accession Number | BC029919 |
| Gene Symbol | SRGAP1 |
| Gene ID (NCBI) | 57522 |
| RRID | AB_2270916 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z6B7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SRGAP1 antibody 13252-1-AP | Download protocol |
| IHC protocol for SRGAP1 antibody 13252-1-AP | Download protocol |
| IP protocol for SRGAP1 antibody 13252-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Am Soc Nephrol SRGAP1 Controls Small Rho GTPases To Regulate Podocyte Foot Process Maintenance. | ||
J Exp Clin Cancer Res srGAP1 mediates the migration inhibition effect of Slit2-Robo1 in colorectal cancer. | ||
Cell Rep A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signaling |
