验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse brain tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22519-1-AP targets SRGAP2 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | SRGAP2 fusion protein Ag18278 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SLIT-ROBO Rho GTPase activating protein 2 |
| 别名 | SRGAP2A, SLIT-ROBO Rho GTPase-activating protein 2, Rho GTPase-activating protein 34, Formin-binding protein 2, FNBP2 |
| 计算分子量 | 1071 aa, 121 kDa |
| 观测分子量 | 121 kDa |
| GenBank蛋白编号 | BC132874 |
| 基因名称 | SRGAP2 |
| Gene ID (NCBI) | 23380 |
| RRID | AB_2879114 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | O75044 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLIT-ROBO Rho GTPase-activating protein 2 (SRGAP2) is also named as ARHGAP34, FNBP2, KIAA0456 and SRGAP2A. SRGAP2 plays important roles in axon guidance, neuronal migration, synapse formation, and nerve regeneration. SRGAP2 protein was first expressed in the retina of normal mice at the embryonic stage and was mainly located in the mature retinal ganglion cell layer and the inner nuclear layer (PMID: 37056152). SRGAP2 plays a key role in neuronal morphogenesis and migration mainly during development of the cerebral cortex (PMID: 20810653, PMID: 27373832, PMID: 28333212). SRGAP2 are critical for neuronal migration through inactivation of Rho GTPases Cdc42, Rac1, and RhoA (PMID: 20810653). SRGAP2A promotes synaptic maturation, and ultimately the synaptic accumulation of AMPA and GABAA receptors, by interacting with key components of both excitatory and inhibitory postsynaptic scaffolds, Homer and Gephyrin (PMID: 27373832).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SRGAP2 antibody 22519-1-AP | Download protocol |
| IHC protocol for SRGAP2 antibody 22519-1-AP | Download protocol |
| IP protocol for SRGAP2 antibody 22519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer
| ||
Nat Commun Haploinsufficiency in the ANKS1B gene encoding AIDA-1 leads to a neurodevelopmental syndrome. | ||
Cell Rep Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton | ||
Biomed Pharmacother CDDO-Me ameliorates podocyte injury through anti-oxidative stress and regulation of actin cytoskeleton in adriamycin nephropathy |
