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验证数据展示

  • WB analysis using 29624-1-AP

    WB analysis using 29624-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29624-1-AP (SRGAP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 29624-1-AP

    IF Staining of mouse brain using 29624-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SRGAP3 antibody (29624-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 29624-1-AP

    IHC staining of human ovary cancer using 29624-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29624-1-AP (SRGAP3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SRGAP3 Polyclonal antibody

SRGAP3 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 29624-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

SRGAP 3, SLIT-ROBO Rho GTPase-activating protein 3, SLIT ROBO Rho GTPase activating protein 3, Rho GTPase-activating protein 14, Mental disorder-associated GAP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29624-1-AP targets SRGAP3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SRGAP3 fusion protein Ag31196 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SLIT-ROBO Rho GTPase activating protein 3
别名 SRGAP 3, SLIT-ROBO Rho GTPase-activating protein 3, SLIT ROBO Rho GTPase activating protein 3, Rho GTPase-activating protein 14, Mental disorder-associated GAP
计算分子量 124 kDa
观测分子量125 kDa
GenBank蛋白编号NM_014850
基因名称 SRGAP3
Gene ID (NCBI) 9901
RRIDAB_2918334
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43295
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRGAP3 (SLIT-ROBO Rho GTPase Activating Protein 3), also named MEGAP and WRP, is a member of the Slit-Robo sub-family of Rho GTPase-activating proteins (Rho GAPs), controls actin and microtubule dynamics through negative regulation of Rac(PMID: 23108406). Diseases associated with SRGAP3 include Pilomyxoid Astrocytoma and Avoidant Personality Disorder. SRGAP3 is highly expressed in fetal and adult brain, including the cortex and the hippocampus.

实验方案

Product Specific Protocols
WB protocol for SRGAP3 antibody 29624-1-APDownload protocol
IHC protocol for SRGAP3 antibody 29624-1-APDownload protocol
IF protocol for SRGAP3 antibody 29624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols