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SRGAP3 Polyclonal antibody

SRGAP3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29624-1-AP

Print datasheet

Synonyms

SRGAP 3, SLIT-ROBO Rho GTPase-activating protein 3, SLIT ROBO Rho GTPase activating protein 3, Rho GTPase-activating protein 14, Mental disorder-associated GAP

验证数据展示

  • WB analysis using 29624-1-AP

    WB analysis using 29624-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29624-1-AP (SRGAP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 29624-1-AP

    IF Staining of mouse brain using 29624-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SRGAP3 antibody (29624-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 29624-1-AP

    IHC staining of human ovary cancer using 29624-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29624-1-AP (SRGAP3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29624-1-AP targets SRGAP3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SRGAP3 fusion protein Ag31196 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SLIT-ROBO Rho GTPase activating protein 3
Synonyms SRGAP 3, SLIT-ROBO Rho GTPase-activating protein 3, SLIT ROBO Rho GTPase activating protein 3, Rho GTPase-activating protein 14, Mental disorder-associated GAP
Calculated Molecular Weight 124 kDa
Observed Molecular Weight125 kDa
GenBank Accession NumberNM_014850
Gene Symbol SRGAP3
Gene ID (NCBI) 9901
RRIDAB_2918334
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43295
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRGAP3 (SLIT-ROBO Rho GTPase Activating Protein 3), also named MEGAP and WRP, is a member of the Slit-Robo sub-family of Rho GTPase-activating proteins (Rho GAPs), controls actin and microtubule dynamics through negative regulation of Rac(PMID: 23108406). Diseases associated with SRGAP3 include Pilomyxoid Astrocytoma and Avoidant Personality Disorder. SRGAP3 is highly expressed in fetal and adult brain, including the cortex and the hippocampus.

实验方案

Product Specific Protocols
WB protocol for SRGAP3 antibody 29624-1-APDownload protocol
IHC protocol for SRGAP3 antibody 29624-1-APDownload protocol
IF protocol for SRGAP3 antibody 29624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols