验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, LNCaP cells, HepG2 cells, PC-3 cells, mouse liver tissue, rat liver tissue, RAW 264.7 cells, mouse brain tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human kidney tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19858-1-AP targets Spermidine synthase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Spermidine synthase fusion protein Ag13936 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spermidine synthase |
| 别名 | SRM, SRML1, SPS1, SPDSY, PAPT |
| 计算分子量 | 302 aa, 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC000309 |
| 基因名称 | Spermidine synthase |
| Gene ID (NCBI) | 6723 |
| RRID | AB_10665555 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P19623 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Spermidine synthase antibody 19858-1-AP | Download protocol |
| IHC protocol for Spermidine synthase antibody 19858-1-AP | Download protocol |
| IF protocol for Spermidine synthase antibody 19858-1-AP | Download protocol |
| IP protocol for Spermidine synthase antibody 19858-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM. | ||
Cancer Sci The miR-1908/SRM regulatory axis contributes to extracellular vesicle secretion in prostate cancer. | ||
J Proteomics Proteomic analysis revealed different responses to hypergravity of soleus and extensor digitorum longus muscles in mice. | ||
Am J Physiol Cell Physiol The Regulation of Polyamine Pathway Proteins in Models of Skeletal Muscle Hypertrophy and Atrophy - a potential role for mTORC1. | ||
Biol Reprod Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation period. |
