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SRMS Polyclonal antibody

SRMS Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26447-1-AP

Print datasheet

Synonyms

C20orf148, SRM, SRMS, Tyrosine protein kinase Srms

验证数据展示

  • IP experiment of HL-60 using 26447-1-AP

    IP experiment of HL-60 using 26447-1-AP

    IP result of anti-SRMS (IP:26447-1-AP, 4ug; Detection:26447-1-AP 1:300) with HL-60 cells lysate 2800ug.

  • IHC staining of human breast cancer using 26447-1-AP

    IHC staining of human breast cancer using 26447-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26447-1-AP (SRMS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 26447-1-AP

    IHC staining of human breast cancer using 26447-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26447-1-AP (SRMS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IP detected inHL-60 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26447-1-AP is SRMS Fusion Protein expressed in E. coli.

Tested Applications IP, IHC, ELISA Application Description
Tested Reactivity human
Immunogen SRMS fusion protein Ag24062 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation sites
Synonyms C20orf148, SRM, SRMS, Tyrosine protein kinase Srms
Calculated Molecular Weight 488 aa, 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC153198
Gene Symbol SRMS
Gene ID (NCBI) 6725
RRIDAB_2880516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3Y6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for SRMS antibody 26447-1-APDownload protocol
IP protocol for SRMS antibody 26447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols