验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, human heart tissue |
| Positive IP detected in | A549 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11528-1-AP targets SRP14 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SRP14 fusion protein Ag2099 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | signal recognition particle 14kDa (homologous Alu RNA binding protein) |
| 别名 | ALURBP, 18 kDa Alu RNA-binding protein, 18 kDa Alu RNA binding protein |
| 计算分子量 | 136 aa, 15 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | BC035495 |
| 基因名称 | SRP14 |
| Gene ID (NCBI) | 6727 |
| RRID | AB_2194708 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P37108 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Signal recognition particle(SRP) acts in three distinct ways: 1 it binds to the signal sequence of the nascent polypeptide to be translocated, which is exposed on the surface of the translating ribosome; 2 it temporarily retards the nascent polypeptide from further elongation; 3 it mediates docking of the SRP-ribosome-nascent polypeptide chain complex to the RER membrane via the heterodimeric SRP-receptor (SR). SRP14, also named as 18 kDa Alu RNA-binding protein, is a 136 amino acid protein. Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9. SRP14 protein is a component of signal-recognition-particle that has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. SRP14 form a heterodimer with SRP9, which recognizes Alu RNA and the related 7SL RNA.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SRP14 antibody 11528-1-AP | Download protocol |
| IF protocol for SRP14 antibody 11528-1-AP | Download protocol |
| IP protocol for SRP14 antibody 11528-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer.
| ||
Mol Cell Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover. | ||
Genes Dev Human intron-encoded Alu RNAs are processed and packaged into Wdr79-associated nucleoplasmic box H/ACA RNPs. | ||
Nucleic Acids Res Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. | ||
Nucleic Acids Res Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle. | ||
