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SRP54 Polyclonal antibody

SRP54 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17976-1-AP

Print datasheet

Synonyms

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4

验证数据展示

  • WB analysis of HeLa using 17976-1-AP

    WB analysis of HeLa using 17976-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17976-1-AP (SRP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 17976-1-AP

    IF Staining of HepG2 using 17976-1-AP

    Immunofluorescent analysis of HepG2 cells, using SRP54 antibody 17976-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of HeLa using 17976-1-AP

    IP experiment of HeLa using 17976-1-AP

    IP result of anti-SRP54 (IP:17976-1-AP, 4ug; Detection:17976-1-AP 1:500) with HeLa cells lysate 1600ug.

  • IHC staining of human breast cancer using 17976-1-AP

    IHC staining of human breast cancer using 17976-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17976-1-AP (SRP54 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 17976-1-AP

    IHC staining of human breast cancer using 17976-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17976-1-AP (SRP54 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17976-1-AP targets SRP54 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen SRP54 fusion protein Ag12166 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal recognition particle 54kDa
Synonyms Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC003389
Gene Symbol SRP54
Gene ID (NCBI) 6729
RRIDAB_2194722
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61011
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM). This antibody specifically recognizes the 54kd SRP54 protein.

实验方案

Product Specific Protocols
WB protocol for SRP54 antibody 17976-1-APDownload protocol
IHC protocol for SRP54 antibody 17976-1-APDownload protocol
IF protocol for SRP54 antibody 17976-1-APDownload protocol
IP protocol for SRP54 antibody 17976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Nucleic Acids Res

Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle.

Authors - Piazzon Nathalie N
View Article