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SRP54 Monoclonal antibody, PBS Only

SRP54 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6D1

Cat no : 67005-1-PBS

Print datasheet

Synonyms

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 1D6D1

验证数据展示

  • WB analysis using 67005-1-Ig (same clone as 67005-1-PBS)

    WB analysis using 67005-1-Ig (same clone as 67005-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67005-1-Ig (SRP54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67005-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67005-1-Ig (same clone as 67005-1-PBS)

    IHC staining of human breast cancer using 67005-1-Ig (same clone as 67005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67005-1-Ig (SRP54 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67005-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67005-1-Ig (same clone as 67005-1-PBS)

    FC experiment of HeLa using 67005-1-Ig (same clone as 67005-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SRP54 (67005-1-Ig, Clone:1D6D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67005-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67005-1-Ig (same clone as 67005-1-PBS)

    IF Staining of HepG2 using 67005-1-Ig (same clone as 67005-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SRP54 antibody (67005-1-Ig, Clone: 1D6D1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67005-1-PBS in a different storage buffer formulation.

产品信息

67005-1-PBS targets SRP54 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SRP54 fusion protein Ag12166 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name signal recognition particle 54kDa
Synonyms Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 1D6D1
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC003389
Gene Symbol SRP54
Gene ID (NCBI) 6729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61011
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).